​OASIS Micro features infinity-corrected optics and accepts 'standard' microscope objective lenses. This system will provide a FoV and output intensity (from a Polygon or a wide-field illuminator) that are similar to when using a commercial microscope (e.g. Nikon, Leica, Olympus or Zeiss). Therefore, OASIS Micro is recommended for applications that require high resolution similar to that of a typical commercial microscope, but at the same time also calls for excellent reconfigurability/flexibility for live animal investigation.

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Translates and Rotates for Live Animal Research
Simultaneously Image & Deliver Wide-field and/or Pattern Illumination
Macro or Microscopic Optical Assembly
Modular Platform Tailors to any Experimental Application
Excellent System for:
In Vivo Optogenetics
Photostimulation
Photoactivation

Mightex's Optical Activation, Stimulation and Imaging System (OASIS) is designed specifically to meet the growing demand to conduct experiments on live animals, when system flexibility is a primary consideration and a large Field of View (FoV) is usually required. In Optogenetics, for example, in vivo patterned illumination on rodents, monkeys and other animals is the next step towards the ultimate goal of being able to record and control the activity of each neuron in the brain of behaving animals at a single cell and ms resolution. To achieve patterned illumination for in vivo applications, the OASIS features Mightex’s market-leadingPolygon400 patterned Illuminator to deliver light to a specified area of the specimen while leaving neighboring areas untouched.

Whereas traditional microscopes are a fixed system with limited flexibility, OASIS is designed to provide excellent reconfigurability/flexibility and can be configured to work around a fixed specimen such as a sedated live animal. A heavy-duty precision XYZ stage is mounted on the support structure and the OASIS optical assembly is in turn mounted on the XYZ stage. The XYZ stage controls the lateral motion as well as focus of the system. A pan/tilt mechanism add-on is also available to orient the OASIS relative to the live animal. Motorized versions of both the XYZ stage and tilt mechanism are available.

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​OASIS Schematic

OASIS Illumination Overview

OASIS features several illumination options that customers can take advantage of. One of the most important options is the option to integrate Mightex’s market leading Polygon400 patterned illuminator to perform in vivo & in vitro patterned illumination options. A wide field illumination option is available where users can select any number of wavelengths between (240nm-940nm) and couple it into OASIS.

In addition to selecting wide-field or patterned illumination options, OASIS is configurable and flexible to incorporate multiple patterned illumination or wide illumination devices to provide maximum versatility possible. To identify the illumination set-up that is ideal for your research our support team will be glad to walk you through the choices.

OASIS Illumination Options

Schematic Shows the Single Patterned Illuminator Option

List of OASIS Illumination Options Single Polygon400 Patterned Illuminator Multi or Single Wavelength Wide-field Illumination setup Polygon400 + Wide-field Setup Dual Polygon400 Setup

Polygon400 Specifications with OASIS

A popular choice for customers is to use Mightex’s market leading Polygon400 with OASIS. Either a Polygon400 with built-in LEDs (E-Series) or with a lightguide input to be used with external LED Sources (G-Series) can be used. Polygon E-Series is suitable for customers requiring only 1-3 wavelengths, while Polygon G-Series is advantageous when customers want to work with their existing light sources or want to use super-high power illuminations sources like Mightex’s Type-H LEDs.

For more information comparing the different Polygon series options please take a look at our Polygon400 overview page here.

Polygon400 Projection Area with OASIS Micro

To calculate illumination area and pixel resolution at the specimen, simply divide the above numbers by the magnification of the objective. For example, under a 10x objective on an Olympus microscope, the illumination area will be 0.78mm x 1.39mm with a pixel resolution of 1.62μm.

Polygon400 Output Power with OASIS Micro

OASIS Compatible Cameras

OASIS Standard configuration features a C-Mount camera mounting which allows OASIS to be used in conjunction with any C-Mount camera. For customers without a camera to use with OASIS Mightex also offers a portfolio of robust CCD Microscopy Cameras that are cost effective imaging solutions with excellent performance. More information about Mightex cameras can be found here.

OASIS Camera Options

		OASIS Camera Compatibility Compatible with all C-Mount Cameras For Mightex Microscopy Cameras Please Click Here
Schematic Shows a Mightex CCD camera mounted on OASIS Micro.   OASIS Compatible Objectives If using OASIS Micro most major commercial microscope objectives will be compatible with the system. Just let our support team know which objective you plan to use and they will make sure to select an optical assembly configuration for the vendor objective. Mightex is also able to provide objectives with the OASIS as an add on as well. Let us know your key requirements and we will be glad to make a recommendation. If using OASIS Macro there are three objectives option that the user can choose between This includes a 4X, 2.35X and 1.9X Macro objectives. OASIS Optical Assembly Options   OASIS LED Controller Options List of OASIS Controller Options BLS-1000/3000/13000 Analog & Manual Controllers BLS-SA/PL Software & TTL Controllers (BLS-PAD-000) + (#2) (BLS Digital I/O Box) + (#1) Schematic Shows a Mightex BLS-SA/PL Controller     OASIS Compatible BioLED Controller Summary Chart    Manual Software Analog TTL BLS-SAxx-US BLS-PLxx-US • • BLS-1000-2 BLS-3000-2 BLS-13000-1 • • BLS-SA/PLxx-US + BLS-PAD-000 • • • BLS-1000/3000/13000 + BLS-IO04-US • • • • For more information about each Mightex BioLED controller please follow the links in the table above.     For more technical details and specifications, please check